Isolation of rat liver hepatocytes
DS Neufeld - Basic Cell Culture Protocols, 1997 - Springer
DS Neufeld
Basic Cell Culture Protocols, 1997•SpringerThe liver is composed of hepatocytes, endothelial, Kupffer, fat-storing, and pit cells. This
chapter describes a technique for isolating hepatocytes, which represent 60–65% of the
cells of the liver and about 80% of the volume of the organ (since they are larger than the
other cells)(1–3). Two years after Howard et. al.(4) discovered that collagenase was a very
important tool in the separation of liver cells, Berry and Friend (5) introduced the technique
of collagenase perfusion of the liver.
chapter describes a technique for isolating hepatocytes, which represent 60–65% of the
cells of the liver and about 80% of the volume of the organ (since they are larger than the
other cells)(1–3). Two years after Howard et. al.(4) discovered that collagenase was a very
important tool in the separation of liver cells, Berry and Friend (5) introduced the technique
of collagenase perfusion of the liver.
Abstract
The liver is composed of hepatocytes, endothelial, Kupffer, fat-storing, and pit cells. This chapter describes a technique for isolating hepatocytes, which represent 60–65% of the cells of the liver and about 80% of the volume of the organ (since they are larger than the other cells) (1–3). Two years after Howard et. al. (4) discovered that collagenase was a very important tool in the separation of liver cells, Berry and Friend (5) introduced the technique of collagenase perfusion of the liver.
Springer
