Nicotinamide, a component of complex culture media, inhibits mouse embryo development in vitro and reduces subsequent developmental potential after transfer
FCH Tsai, DK Gardner - Fertility and sterility, 1994 - Elsevier
Objective To determine the effects of B-group vitamins present in culture media on mouse
embryo development in vitro and subsequent viability. Design Mouse zygotes were cultured
in the presence of B-group vitamins. Embryo morphology and cell numbers were determined
at 96 and 120 hours after hCG. Viability was assessed by transfer of embryos after 3 days of
culture to pseudopregnant recipients. Resultant pregnancy rates (PRs) and fetal weights
were determined. Results Supplementation of an amino acid-free medium with minimal …
embryo development in vitro and subsequent viability. Design Mouse zygotes were cultured
in the presence of B-group vitamins. Embryo morphology and cell numbers were determined
at 96 and 120 hours after hCG. Viability was assessed by transfer of embryos after 3 days of
culture to pseudopregnant recipients. Resultant pregnancy rates (PRs) and fetal weights
were determined. Results Supplementation of an amino acid-free medium with minimal …
