[HTML][HTML] Mapping receptor binding sites in interleukin (IL)-1 receptor antagonist and IL-1β by site-directed mutagenesis: identification of a single site in IL-1Ra and two …
RJ Evans, J Bray, JD Childs, GPA Vigers… - Journal of Biological …, 1995 - ASBMB
Interleukin-1 receptor antagonist (IL-1ra), an IL-1 family member, binds with high affinity to
the type I IL-1 receptor (IL-1RI), blocking IL-1 binding but not inducing an IL-1-like response.
Extensive site-directed mutagenesis has been used to identify residues in IL-1ra and IL-1β
involved in binding to IL-1RI. These analyses have revealed the presence of two discrete
receptor binding sites on IL-1β. Only one of these sites is present on IL-1ra, consisting of
residues Trp-16, Gln-20, Tyr-34, Gln-36, and Tyr-147. Interestingly, the absent second site is …
the type I IL-1 receptor (IL-1RI), blocking IL-1 binding but not inducing an IL-1-like response.
Extensive site-directed mutagenesis has been used to identify residues in IL-1ra and IL-1β
involved in binding to IL-1RI. These analyses have revealed the presence of two discrete
receptor binding sites on IL-1β. Only one of these sites is present on IL-1ra, consisting of
residues Trp-16, Gln-20, Tyr-34, Gln-36, and Tyr-147. Interestingly, the absent second site is …