Detection of picomole levels of hydroperoxides using a fluorescent dichlorofluorescein assay
R Cathcart, E Schwiers, BN Ames - Analytical biochemistry, 1983 - Elsevier
R Cathcart, E Schwiers, BN Ames
Analytical biochemistry, 1983•ElsevierA highly sensitive fluorometric method for the quantitation of cholesterol, lipid, and other
hydroperoxides at the picomole level is described. The method is based on the oxidation of
dichlorofluoroscin to the fluorescent dichlorofluoroscein by hydroperoxide and hematin
under mild conditions. A 1: 1 stoichiometry is observed between the hydroperoxide added
and the dichlorofluoroscein produced. Since endoperoxides (eg, PGH2) do not react in the
assay, they do not interfere in the determination of lipid hydroperoxides.
hydroperoxides at the picomole level is described. The method is based on the oxidation of
dichlorofluoroscin to the fluorescent dichlorofluoroscein by hydroperoxide and hematin
under mild conditions. A 1: 1 stoichiometry is observed between the hydroperoxide added
and the dichlorofluoroscein produced. Since endoperoxides (eg, PGH2) do not react in the
assay, they do not interfere in the determination of lipid hydroperoxides.
A highly sensitive fluorometric method for the quantitation of cholesterol, lipid, and other hydroperoxides at the picomole level is described. The method is based on the oxidation of dichlorofluoroscin to the fluorescent dichlorofluoroscein by hydroperoxide and hematin under mild conditions. A 1:1 stoichiometry is observed between the hydroperoxide added and the dichlorofluoroscein produced. Since endoperoxides (e.g., PGH2) do not react in the assay, they do not interfere in the determination of lipid hydroperoxides.
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