[HTML][HTML] Deficiency of a transmembrane prolyl 4-hydroxylase in the zebrafish leads to basement membrane defects and compromised kidney function
Prolyl 4-hydroxylases (P4Hs) catalyze the hydroxylation of collagens and hypoxia-inducible
factor (HIF)-α subunits. We studied the zebrafish homologue of the recently characterized
human transmembrane P4H (P4H-TM) that can hydroxylate HIF-α, but not collagens, in vitro
and influence HIF-α levels in cellulo. The zebrafish P4H-TM mRNA had its highest
expression in the eye and brain and lower levels in other tissues, including the kidney.
Morpholino knockdown of P4H-TM in embryos resulted in a reduction in the size of the eye …
factor (HIF)-α subunits. We studied the zebrafish homologue of the recently characterized
human transmembrane P4H (P4H-TM) that can hydroxylate HIF-α, but not collagens, in vitro
and influence HIF-α levels in cellulo. The zebrafish P4H-TM mRNA had its highest
expression in the eye and brain and lower levels in other tissues, including the kidney.
Morpholino knockdown of P4H-TM in embryos resulted in a reduction in the size of the eye …