[PDF][PDF] The small GTPase Rac2 controls phagosomal alkalinization and antigen crosspresentation selectively in CD8+ dendritic cells
A Savina, A Peres, I Cebrian, N Carmo, C Moita… - Immunity, 2009 - cell.com
Immunity, 2009•cell.com
A unique subpopulation of spleen dendritic cells (DCs) that express the CD8 surface marker
efficiently present phagocytosed antigens to CD8+ T lymphocytes in a process called"
crosspresentation," which initiates cytotoxic immune responses. We now show that the small
GTPase Rac2 plays a critical role in antigen crosspresentation selectively in this DC
subpopulation. In CD8+ DCs, Rac2 determines the subcellular assembly of the NADPH
oxidase complex (NOX2) to phagosomes, whereas in CD8− DCs, Rac1 mediates the …
efficiently present phagocytosed antigens to CD8+ T lymphocytes in a process called"
crosspresentation," which initiates cytotoxic immune responses. We now show that the small
GTPase Rac2 plays a critical role in antigen crosspresentation selectively in this DC
subpopulation. In CD8+ DCs, Rac2 determines the subcellular assembly of the NADPH
oxidase complex (NOX2) to phagosomes, whereas in CD8− DCs, Rac1 mediates the …
Summary
A unique subpopulation of spleen dendritic cells (DCs) that express the CD8 surface marker efficiently present phagocytosed antigens to CD8+ T lymphocytes in a process called "crosspresentation," which initiates cytotoxic immune responses. We now show that the small GTPase Rac2 plays a critical role in antigen crosspresentation selectively in this DC subpopulation. In CD8+ DCs, Rac2 determines the subcellular assembly of the NADPH oxidase complex (NOX2) to phagosomes, whereas in CD8− DCs, Rac1 mediates the assembly of NOX2 at the plasma membrane. In the absence of Rac2, the production of reactive oxygen species (ROS) in DC-phagosomes was abolished, the phagosomal pH dropped, and the efficiency of antigen crosspresentation was reduced. We conclude that the activity of Rac1 and 2 control crosspresentation in DC subpopulations through the regulation of phagosomal oxidation and pH.
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