[HTML][HTML] Indoleamine 2, 3-dioxygenase (IDO) is expressed at feto-placental unit throughout mouse gestation: An immunohistochemical study
S Hemmati, M Jeddi-Tehrani, E Torkabadi… - … of Reproduction & …, 2009 - ncbi.nlm.nih.gov
S Hemmati, M Jeddi-Tehrani, E Torkabadi, J Ghassemi, P Danesh, LB Yarmohammadi…
Journal of Reproduction & Infertility, 2009•ncbi.nlm.nih.govMaterials and Methods Syngeneic pregnant mice were examined for vaginal plug to
discover about their state of pregnancy. A total of three pregnant mice were examined at
each stage. The examination was further confirmed by the detection of sperm in vaginal
smear. On the gestational days of 2 nd, 12 th and 18 th, the uterus and oviduct were
removed and expression of IDO was investigated in the endometrium, placenta and oviduct
by immunohistochemistry. Results Our results showed that IDO is expressed consistently in …
discover about their state of pregnancy. A total of three pregnant mice were examined at
each stage. The examination was further confirmed by the detection of sperm in vaginal
smear. On the gestational days of 2 nd, 12 th and 18 th, the uterus and oviduct were
removed and expression of IDO was investigated in the endometrium, placenta and oviduct
by immunohistochemistry. Results Our results showed that IDO is expressed consistently in …
Materials and Methods
Syngeneic pregnant mice were examined for vaginal plug to discover about their state of pregnancy. A total of three pregnant mice were examined at each stage. The examination was further confirmed by the detection of sperm in vaginal smear. On the gestational days of 2 nd, 12 th and 18 th, the uterus and oviduct were removed and expression of IDO was investigated in the endometrium, placenta and oviduct by immunohistochemistry.
Results
Our results showed that IDO is expressed consistently in feto-maternal interface throughout pregnancy. In endometrium, expression of IDO was predominantly confined to luminal and glandular epithelial cells. Cells at junctional and labyrinth zones of placenta showed strong IDO immunoreactivity as well.
Conclusion
Expression of IDO at the protein level in reproductive tract of pregnant mice during entire periods of gestation points to its potential protective role in maintenance of pregnancy. In our knowledge this is the first report of expression of IDO in feto-maternal phase during murine pregnancy.
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