Single-nucleus RNA sequencing identifies new classes of proximal tubular epithelial cells in kidney fibrosis
YA Lu, CT Liao, R Raybould, B Talabani… - Journal of the …, 2021 - journals.lww.com
Background Proximal tubular cells (PTCs) are the most abundant cell type in the kidney.
PTCs are central to normal kidney function and to regeneration versus organ fibrosis
following injury. This study used single-nucleus RNA sequencing (snRNAseq) to describe
the phenotype of PTCs in renal fibrosis. Methods Kidneys were harvested from naïve mice
and from mice with renal fibrosis induced by chronic aristolochic acid administration. Nuclei
were isolated using Nuclei EZ Lysis buffer. Libraries were prepared on the 10× platform, and …
PTCs are central to normal kidney function and to regeneration versus organ fibrosis
following injury. This study used single-nucleus RNA sequencing (snRNAseq) to describe
the phenotype of PTCs in renal fibrosis. Methods Kidneys were harvested from naïve mice
and from mice with renal fibrosis induced by chronic aristolochic acid administration. Nuclei
were isolated using Nuclei EZ Lysis buffer. Libraries were prepared on the 10× platform, and …