Epithelial vasopressin type-2 receptors regulate myofibroblasts by a YAP-CCN2–dependent mechanism in polycystic kidney disease
Background Fibrosis is a major cause of loss of renal function in autosomal dominant
polycystic kidney disease (ADPKD). In this study, we examined whether vasopressin type-2
receptor (V2R) activity in cystic epithelial cells can stimulate interstitial myofibroblasts and
fibrosis in ADPKD kidneys. Methods We treated Pkd1 gene knockout (Pkd1KO) mice with
dDAVP, a V2R agonist, for 3 days and evaluated the effect on myofibroblast deposition of
extracellular matrix (ECM). We also analyzed the effects of conditioned media from primary …
polycystic kidney disease (ADPKD). In this study, we examined whether vasopressin type-2
receptor (V2R) activity in cystic epithelial cells can stimulate interstitial myofibroblasts and
fibrosis in ADPKD kidneys. Methods We treated Pkd1 gene knockout (Pkd1KO) mice with
dDAVP, a V2R agonist, for 3 days and evaluated the effect on myofibroblast deposition of
extracellular matrix (ECM). We also analyzed the effects of conditioned media from primary …