A defect in a novel Nek-family kinase causes cystic kidney disease in the mouse and in zebrafish
2002•journals.biologists.com
The murine autosomal recessive juvenile cystic kidney (jck) mutation results in polycystic
kidney disease. We have identified in jck mice a mutation in Nek8, a novel and highly
conserved member of the Nek kinase family. In vitro expression of mutated Nek8 results in
enlarged, multinucleated cells with an abnormal actin cytoskeleton. To confirm that a defect
in the Nek8 gene can cause cystic disease, we performed a cross-species analysis: injection
of zebrafish embryos with a morpholino anti-sense oligonucleotide corresponding to the …
kidney disease. We have identified in jck mice a mutation in Nek8, a novel and highly
conserved member of the Nek kinase family. In vitro expression of mutated Nek8 results in
enlarged, multinucleated cells with an abnormal actin cytoskeleton. To confirm that a defect
in the Nek8 gene can cause cystic disease, we performed a cross-species analysis: injection
of zebrafish embryos with a morpholino anti-sense oligonucleotide corresponding to the …
The murine autosomal recessive juvenile cystic kidney (jck)mutation results in polycystic kidney disease. We have identified injck mice a mutation in Nek8, a novel and highly conserved member of the Nek kinase family. In vitro expression of mutated Nek8results in enlarged, multinucleated cells with an abnormal actin cytoskeleton. To confirm that a defect in the Nek8 gene can cause cystic disease,we performed a cross-species analysis: injection of zebrafish embryos with a morpholino anti-sense oligonucleotide corresponding to the ortholog ofNek8 resulted in the formation of pronephric cysts. These results demonstrate that comparative analysis of gene function in different model systems represents a powerful means to annotate gene function.
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