[HTML][HTML] Novel Anti-carbohydrate Antibodies Reveal the Cooperative Function of Sulfated N-and O-Glycans in Lymphocyte Homing*♦
J Hirakawa, K Tsuboi, K Sato, M Kobayashi… - Journal of biological …, 2010 - ASBMB
Cell surface glycans play pivotal roles in immune cell trafficking and immunity. Here we
present an efficient method for generating anti-carbohydrate monoclonal antibodies (mAbs)
using gene-targeted mice and describe critical glycans in lymphocyte homing. We
immunized sulfotransferase GlcNAc6ST-1 and GlcNAc6ST-2 doubly deficient mice with
sulfotransferase-overexpressing Chinese hamster ovary cells and generated two mAbs,
termed S1 and S2. Both S1 and S2 bound high endothelial venules (HEVs) in the lymphoid …
present an efficient method for generating anti-carbohydrate monoclonal antibodies (mAbs)
using gene-targeted mice and describe critical glycans in lymphocyte homing. We
immunized sulfotransferase GlcNAc6ST-1 and GlcNAc6ST-2 doubly deficient mice with
sulfotransferase-overexpressing Chinese hamster ovary cells and generated two mAbs,
termed S1 and S2. Both S1 and S2 bound high endothelial venules (HEVs) in the lymphoid …