[HTML][HTML] In vivo characterization of a thioredoxin h target protein defines a new peroxiredoxin family
Disruption of the two thioredoxin genes in yeast dramatically affects cell viability and growth.
Expression ofArabidopsis thioredoxin AtTRX3 in theSaccharomyces thioredoxin Δ strain
EMY63 restores a wild-type cell cycle, the ability to grow on methionine sulfoxide, and H 2 O
2 tolerance. In order to isolate thioredoxin targets related to these phenotypes, we prepared
a C35S (Escherichia coli numbering) thioredoxin mutant to stabilize the intermediate
disulfide bridged complex and we added a polyhistidine N-terminal extension in order to …
Expression ofArabidopsis thioredoxin AtTRX3 in theSaccharomyces thioredoxin Δ strain
EMY63 restores a wild-type cell cycle, the ability to grow on methionine sulfoxide, and H 2 O
2 tolerance. In order to isolate thioredoxin targets related to these phenotypes, we prepared
a C35S (Escherichia coli numbering) thioredoxin mutant to stabilize the intermediate
disulfide bridged complex and we added a polyhistidine N-terminal extension in order to …