[HTML][HTML] S-nitrosoglutathione is cleaved by the thioredoxin system with liberation of glutathione and redox regulating nitric oxide
D Nikitovic, A Holmgren - Journal of Biological Chemistry, 1996 - ASBMB
In activated human neutrophils a burst of nitric oxide (NO) converts intracellular GSH to S-
nitrosoglutathione (GSNO) which is subsequently cleaved to restore GSH by an unknown
mechanism. We discovered that GSNO is an NADPH oxidizing substrate for human or calf
thymus thioredoxin reductase (TR) with an apparent K m value of 60 µM and a K cat of 0.6×
s− 1. Addition of human thioredoxin (Trx) stimulated the initial NADPH oxidation rate
severalfold but was accompanied by progressive inactivation of TR. Escherichia coli TR …
nitrosoglutathione (GSNO) which is subsequently cleaved to restore GSH by an unknown
mechanism. We discovered that GSNO is an NADPH oxidizing substrate for human or calf
thymus thioredoxin reductase (TR) with an apparent K m value of 60 µM and a K cat of 0.6×
s− 1. Addition of human thioredoxin (Trx) stimulated the initial NADPH oxidation rate
severalfold but was accompanied by progressive inactivation of TR. Escherichia coli TR …