Reparixin, an inhibitor of CXCR1 and CXCR2 receptor activation, attenuates blood pressure and hypertension-related mediators expression in spontaneously …
HY Kim, JH Choi, YJ Kang, SY Park… - Biological and …, 2011 - jstage.jst.go.jp
HY Kim, JH Choi, YJ Kang, SY Park, HC Choi, HS Kim
Biological and Pharmaceutical Bulletin, 2011•jstage.jst.go.jpMATERIALS AND METHODS Reagents The trizol reagent for total RNA isolation was
purchased from Invitrogen (Carlsbad, CA, USA). Phosphate-buffered saline (PBS),
Dulbecco's modified Eagle's medium (DMEM), penicillin–streptomycin and fetal bovine
serum (FBS) were purchased from Gibco/BRL (Life Technologies, Gaithersburg, MD, USA).
Recombinant human CXCL8 was purchased from R&D systems (Minneapolis, MN, USA).
Oligonucleotide primers for CCL2, CXCL8, 12-LO, ET-1, Angiotensin II (Ang II) subtype 1 …
purchased from Invitrogen (Carlsbad, CA, USA). Phosphate-buffered saline (PBS),
Dulbecco's modified Eagle's medium (DMEM), penicillin–streptomycin and fetal bovine
serum (FBS) were purchased from Gibco/BRL (Life Technologies, Gaithersburg, MD, USA).
Recombinant human CXCL8 was purchased from R&D systems (Minneapolis, MN, USA).
Oligonucleotide primers for CCL2, CXCL8, 12-LO, ET-1, Angiotensin II (Ang II) subtype 1 …
MATERIALS AND METHODS
Reagents The trizol reagent for total RNA isolation was purchased from Invitrogen (Carlsbad, CA, USA). Phosphate-buffered saline (PBS), Dulbecco’s modified Eagle’s medium (DMEM), penicillin–streptomycin and fetal bovine serum (FBS) were purchased from Gibco/BRL (Life Technologies, Gaithersburg, MD, USA). Recombinant human CXCL8 was purchased from R&D systems (Minneapolis, MN, USA). Oligonucleotide primers for CCL2, CXCL8, 12-LO, ET-1, Angiotensin II (Ang II) subtype 1 receptor (AT1R), Ang II subtype 2 receptor (AT2R), and b-actin polymerase chain reaction (PCR) were synthesized by Bionics (Seoul, Korea). LightCycler FastStart DNA SYBR Green I
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