[HTML][HTML] Adherence-dependent calcium signaling in monocytes: induction of a CD14-high phenotype, stimulus-responsive subpopulation
J Bernardo, AM Billingslea, MF Ortiz, KF Seetoo… - Journal of …, 1997 - Elsevier
Isolation of monocytes by plastic adherence alters cell morphology and function. In order to
study the effects of cell isolation procedures and subsequent culture on monocyte function,
we examined cytoplasmic calcium concentration changes (Δ [Ca2+] in) in human monocytes
isolated by either negative (magnetic bead) or positive (plastic adherence) selection then
stimulated with formyl–Met–Leu–Phe (fMLP), either immediately after isolation, or after 48 h
in culture. We have previously shown that fresh adherence-isolated monocytes respond to …
study the effects of cell isolation procedures and subsequent culture on monocyte function,
we examined cytoplasmic calcium concentration changes (Δ [Ca2+] in) in human monocytes
isolated by either negative (magnetic bead) or positive (plastic adherence) selection then
stimulated with formyl–Met–Leu–Phe (fMLP), either immediately after isolation, or after 48 h
in culture. We have previously shown that fresh adherence-isolated monocytes respond to …